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KMID : 0363320040250010028
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Journal of Korean Oriental Internal Medicine
2004 Volume.25 No. 1 p.28 ~ p.45
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The Effects of Injinchunggan-tang(Yinchenqinggan-tang) on TNF-alpha signal transmission system in HepG2 cell
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Kang Woo-Sung
Kim Young-Chul
Lee Jang-Hoon
Woo Hong-Jung
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Abstract
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Objectives : The main purpose of this study is to evaluate the effect of Injinchunggan-tang on TNF-{alpha} signal transmission system.
Materials and Methods : We analyzed the following with quantitative RT-PCR method; the effect of Injinchunggan-tang on secretion of TNF-alpha mRNA/protein and stability, the effect on gene revelation that consists of signal transmission system (TRAIL, NIK, A20, TRADD, RAIDD, RIP TNFR-I, TNFR-II, TRAF1, TRAF2, FADD), the one on activation of p38, Erk1/2 MAPK and the rate of nuclear NF-{kappa}B/cytosolic;NF-{kappa}B in HepG2 cell. We also analyzed the inhibitory effect of Injinchunggan-tang on the apoptosis of HepG2 cell that TNF-{alpha} induces and the NF-{kappa}B restraint effected by transfection of I{kappa}B{Delta}N through tryphan blue exclusion assay.
Results : Injinchunggan-tang prohibits revelation of TNF-{alpha} mRNA in HepG2 cell and the creation of protein. However, it has no effect on the stability of TNF-{alpha} mRNA. While it did not have any effect on the generation of TRAIL, NIK, A20, TRADD, RAIDD and RIP genes, Injinchunggan-tang reduces the revelation of TNFR-I, TNFR-II, TRAF1, TRAF2 and FADD genes. It has been confirmed that Injinchunggan-tang restraints the revelation of TNF-{alpha} mRNA that is promoted by ethanol, acetaldehyde, lipopolysaccharide, in proportion to the treatment density and time. It activated NF-{kappa}B of HepG2 cell and promoted activation of NF-{kappa}B that is occurred by TNF-{alpha}. It has been observed that the restraint effect against the TNF-{alpha} inducing apoptosis is lost when it is intercepted the function of NF-{kappa}B in HepG2 cell.
Conclusion: It has been confirmed that Injinchunggan-tang has restraining effect against the revelation of TNF-{alpha} and mRNA that is constituent element of TNF-a signal transmission system. It also has been revealed that it restraints the activation of p38, Erk1/2 by TNF-{alpha}. Through this prohibiting effect, it is inferred that it restraints signal transmission among various cells that are related to inflammation reaction. Meanwhile, Injinchunggan-tang protects liver cell from apoptosis that is caused by TNF-{alpha}, by maintaining the activating function for NF-{kappa}B.
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KEYWORD
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Injinchunggan-tang(Yinchenqinggan-tang), TNF-{alpha}, HepG2 cell
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